Wilms Tumor 1 (WT1) Antikörper

Antigen: Wilms Tumor 1 (WT1)

Synonyme: GUD, WAGR, WT33, WIT-2, WT1, WT, wt1-A, xWT1, CWT1, WT-1, wt1, MGC152697, zgc:152697, si:ch211-160j6.3, gud, wagr, wt-1, wt33, xwt1, wit-2

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185674

Produktbeschreibung

Weitere Bezeichnung: Wilms tumor 1 / WT1

Gen-ID: 7490

Immunogen: QDPASTCVPEPASQH

Format: Affinity-purified

Beschreibung: All names and symbols: WT1, Wilms tumor 1, GUD, WAGR, WIT-2, WT33 . Official Symbol: WT1. RefSeq number(s): NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3

Spezifität: This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3). Human 497 aa, 514 aa, 500 aa, 517 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 55kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3kDa according to NP_000369.3). Recommended concentration: 1-3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185674 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Rao, Pham, Imam et al.: "Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis." in: Genes & development, Vol. 20, Issue 2, pp. 147-52, 2006 (PubMed).