CNBP Antikörper (C-Term)

Produktnummer ABIN185618

Der Ziege Polyklonal anti-CNBP Antikörper wird verwendet zum Nachweis von CNBP in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IF und FACS.

Kurzübersicht für CNBP Antikörper (C-Term) (ABIN185618)

Target: CNBP (CCHC-Type Zinc Finger, Nucleic Acid Binding Protein (CNBP))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CNBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-CNBP Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ZNF9 / CNBP

Sequenz: GESGHLAREC TIE

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence GESGHLARECTIE, from the C Terminus of the protein sequence according to NP_003409.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 20 kDa band observed in Human Skeletal Muscle lysates and in lysates of cell lines Daudi, MCF7, MOLT-4 and Neuro-2a (calculated MW of 19.5 kDa according to Human NP_003409.1 and Mouse NP_038521.1). Recommended concentration: 0.3-1 μg/mL

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in MCF7 and Neuro-2a cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CNBP

Target: CNBP (CCHC-Type Zinc Finger, Nucleic Acid Binding Protein (CNBP))

Andere Bezeichnung: CNBP

Hintergrund: ZNF9, CNBP, zinc finger protein 9 (a cellular retroviral nucleic acid binding protein) , HGNC:13164, CNBP1, DM2, PROMM, RNF163 , Proximal myotonic myopathy nucleic acid binding protein, zinc finger protein 273, zinc finger protein 9

Gen-ID: 7555

NCBI Accession: NP_003409