phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) Antikörper

Antigen: phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK)

Synonyme: DBI, IBP, MBR, PBR, BZRP, PKBS, PTBR, mDRC, pk18, PEPCK, PEPCK2, PEPCK-M, 1810010O14Rik, 9130022B02Rik, PCK2, pck, zgc:77867, id:ibd5157, ppc, SCE66.06c, pepC, pckA

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185483

Produktbeschreibung

Weitere Bezeichnung: PCK2 / PEPCK-M

Gen-ID: 5106

Immunogen: KPWKPGDKEPCAH

Format: Affinity-purified

Beschreibung: All names and symbols: PCK2, PEPCK-M, phosphoenolpyruvate carboxykinase 2 (mitochondrial), HGNC:8725, PEPCK, PEPCK2 , PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase. Official Symbol: PCK2. RefSeq number(s): NP_004554.2. Gene ontology terms: obesity

Spezifität: This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1. Human, Mouse, Rat, Dog, Cow

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 75kDa band observed in human liver lysates (calculated MW of 70.7kDa according to NP_004554.2). Recommended concentration: 0.5-1.0 µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185483 (0.5µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Duplus, Benelli, Reis et al.: "Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes." in: Biochimie, Vol. 85, Issue 12, pp. 1257-64, 2004 (PubMed).