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Antikörper
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anti-POU Class 5 Homeobox 1 (POU5F1) Antikörper
POU Class 5 Homeobox 1 (POU5F1) Antikörper
| Antigen |
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| Synonyme |
OCT3, OCT4, OTF3, OTF4, MGC22487, POU5F1, POU5F1L |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (76), Maus (30), Ratte (Rattus) (11), Rind (Kuh) (9), Schwein (7), Affe (6), Hund (5), Schimpanse (2), Pferd (2), Kaninchen (2), Primate (1)
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Applikation
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Western Blot (WB) (66), Immunhistochemie (Paraffinschnitte) (IHC (p)) (21), Enzyme Immunoassay (EIA) (20), Immunfluoreszenz (IF) (12), ELISA (11), Durchflusszytometrie (FACS) (8), Immunpräzipitation (IP) (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Dot Blot (Dot) (3), Immunhistochemie (IHC) (3), Immunzytochemie (ICC) (1)
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| Produktnummer |
ABIN185438 |
| Menge |
100 ug (Varianten) |
| Preis |
286,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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OCT4 / POU5F1
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Gen-ID
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5460, 18999 (mouse), 294562 (rat)
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Immunogen
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C-VTTLGSPMHSN
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Format
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Affinity-purified
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Beschreibung
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All names and symbols: POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3. Official Symbol: POU5F1. RefSeq number(s): NP_002692.2, NP_976034.2
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Spezifität
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This antibody is expected to recognise both reported isoforms (NP_002692 and NP_976034) Human, Mouse, Rat, Dog
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Applikationshinweise
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 38kDa band observed in Human Liver lysates (calculated MW of 38.6kDa according to NP_002692.2). Recommended concentration: 1-3µg/ml.
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Reinigung
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Lagerung
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Jones, Ulbright, Eble et al.: "OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma." in: The American journal of surgical pathology, Vol. 28, Issue 7, pp. 935-40, 2004 (PubMed).
Chew, Loh, Zhang et al.: "Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells." in: Molecular and cellular biology, Vol. 25, Issue 14, pp. 6031-46, 2005 (PubMed).
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Alternativen zu Antigen "POU Class 5 Homeobox 1 (POU5F1)", Typ "Antikörper" finden
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Wirte
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Kaninchen (56), Maus (18), Ratte (8), Ziege (7)
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Reaktivitäten
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Human (76), Maus (30), Ratte (Rattus) (11), Rind (Kuh) (9), Schwein (7), Affe (6), Hund (5), Schimpanse (2), Pferd (2), Kaninchen (2), Primate (1)
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Applikationen
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Western Blot (WB) (66), Immunhistochemie (Paraffinschnitte) (IHC (p)) (21), Enzyme Immunoassay (EIA) (20), Immunfluoreszenz (IF) (12), ELISA (11), Durchflusszytometrie (FACS) (8), Immunpräzipitation (IP) (5), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Dot Blot (Dot) (3), Immunhistochemie (IHC) (3), Immunzytochemie (ICC) (1)
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Konjugate
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Alexa Fluor 488 (2), Alexa Fluor 647 (2), PE (2), Biotin (1), HRP (1)
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Epitope
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N-Term (2), AA 100-200 (1), AA 230-244 (1), E125 (1), transcript variant 1 (1)
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Alternativen 
