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Secreted phosphoprotein 1 (SPP1) Antikörper
| Antigen | Secreted phosphoprotein 1 (SPP1) |
| Synonyme | OPN, BNSP, BSPI, ETA-1, MGC110940, OP, Bsp, Eta, Opn, Ric, Opnl, Apl-1, Spp-1, AA960535, AI790405, OSP, SPP1, spp1, MGC111821 |
| Klonalität | Polyklonal |
| Wirt |
 Alternativen Ziege |
| Reaktivität |
Alternativen Human |
| Applikation |
Alternativen ELISA, Western Blot (WB)
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1 Publikation vorhanden |
| Produktnummer | ABIN185425 |
| Menge | 100 ug |
| Preis | 286,00 € Zzgl. Versandkosten €40,00 und MWSt |
| Lieferung nach |
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| Verfügbarkeit | Lieferung in 5 Werktagen |
Produktbeschreibung
| Weitere Bezeichnung | Osteopontin / SPP1 |
| Gen-ID | 6696, 20750 (mouse) |
| Immunogen | C-DPKSKEEDKHLK |
| Format | Affinity-purified |
| Beschreibung | All names and symbols: SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN , Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1. Official Symbol: SPP1. RefSeq number(s): NP_000573.1 |
| Spezifität | Human, Mouse, Rat, Dog |
Anwendungen
| Applikationshinweise | Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 1-3µg/ml. |
| Reinigung | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Lagerung | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Forschungsgebiet | Hormone, Enzyme, Metabolismus, Extrazelluläre Matrix |
| Beschränkungen | Nur für Forschungszwecke einsetzbar |
Bilder
| ABIN185425 staining (1µg/ml) of MOLT4 cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publikationen
| Publikationen |
Kolb, Kleeff, Guweidhi et al.: "Osteopontin influences the invasiveness of pancreatic cancer cells and is increased in neoplastic and inflammatory conditions." in: Cancer biology & therapy, Vol. 4, Issue 7, pp. 740-6, 2005 (PubMed).
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Alternativen
Alternativen zu Antigen "Secreted phosphoprotein 1 (SPP1)", Typ "Antikörper" finden
| Wirte | Kaninchen (60), Maus (49), Ziege (9) |
| Reaktivitäten | Human (90), Maus (37), Ratte (Rattus) (29), Hund (13), Schwein (8), Kaninchen (2), Rind (Kuh) (1) |
| Applikationen | Western Blot (WB) (96), Enzyme Immunoassay (EIA) (55), Immunhistochemie (Paraffinschnitte) (IHC (p)) (29), ELISA (19), Durchflusszytometrie (FACS) (15), Immunfluoreszenz (IF) (14), Immunhistochemie (IHC) (11), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Immunpräzipitation (IP) (3), Immunzytochemie (ICC) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Neutralisierung (Neut) (1) |
| Konjugate | Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1) |
| Epitope | C-Term (7), AA 164-179 (5), AA 59-74 (5), N-Term (5), AA 0-17 (3) |
