»
Antikörper
»
anti-Osteopontin SPP1
Osteopontin (SPP1) Antikörper
| Antigen |
|
| Synonyme |
OPN, BNSP, BSPI, ETA-1, MGC110940 |
| Klonalität |
Polyklonal |
|
Wirt
|
|
|
Reaktivität
|
|
|
Applikation
|
Western Blot (WB) (81), Enzyme Immunoassay (EIA) (48), Immunhistochemie (Paraffinschnitte) (IHC (p)) (19), ELISA (16), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Immunhistochemie (IHC) (7), Durchflusszytometrie (FACS) (2), Immunzytochemie (ICC) (2), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunfluoreszenz (IF) (1), Immunpräzipitation (IP) (1), Neutralisierung (Neut) (1)
|
| Produktnummer |
ABIN185425 |
| Menge |
100 ug |
| Preis |
286,00 € Zzgl. Versandkosten €20,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferbar innerhalb von 5 Werktagen |
|
Weitere Bezeichnung
|
Osteopontin / SPP1
|
|
Gen-ID
|
6696, 20750 (mouse)
|
|
Immunogen
|
C-DPKSKEEDKHLK
|
|
Format
|
Affinity-purified
|
|
Beschreibung
|
All names and symbols: SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN , Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1. Official Symbol: SPP1. RefSeq number(s): NP_000573.1
|
|
Spezifität
|
Human, Mouse, Rat, Dog
|
|
Applikationshinweise
|
Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 1-3µg/ml.
|
|
Reinigung
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Lagerung
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Forschungsgebiet
|
Hormone, Enzyme, Metabolismus, Extrazelluläre Matrix
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
|
Publikationen
|
Kolb, Kleeff, Guweidhi et al.: "Osteopontin influences the invasiveness of pancreatic cancer cells and is increased in neoplastic and inflammatory conditions." in: Cancer biology & therapy, Vol. 4, Issue 7, pp. 740-6, 2005 (PubMed).
|
Alternativen zu Antigen "Osteopontin (SPP1)", Typ "Antikörper" finden
|
Wirte
|
Maus (47), Kaninchen (36), Ziege (6)
|
|
Reaktivitäten
|
Human (80), Maus (18), Ratte (Rattus) (13), Hund (8), Schwein (6), Kaninchen (1)
|
|
Applikationen
|
Western Blot (WB) (81), Enzyme Immunoassay (EIA) (48), Immunhistochemie (Paraffinschnitte) (IHC (p)) (19), ELISA (16), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Immunhistochemie (IHC) (7), Durchflusszytometrie (FACS) (2), Immunzytochemie (ICC) (2), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunfluoreszenz (IF) (1), Immunpräzipitation (IP) (1), Neutralisierung (Neut) (1)
|
|
Epitope
|
AA 0-17 (3), AA 164-179 (3), AA 59-74 (3), C-Term (2)
|