Cytochrome B Reductase 1 (CYBRD1) Antikörper

Antigen: Cytochrome B Reductase 1 (CYBRD1)

Synonyme: DCYTB, FRRS3, FLJ23462, MGC82810, MGC122108, im:6900453, zgc:110065, CYTB, DKFZp469G0913, Dcytb, 2210407P13Rik

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN185343

Produktbeschreibung

Weitere Bezeichnung: Cytochrome b reductase 1

Gen-ID: 79901

Immunogen: CRNLALDEAGQRSTM

Format: Affinity-purified

Beschreibung: All names and symbols: CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b. Official Symbol: CYBRD1. RefSeq number(s): NP_079119. Gene ontology terms: electron transport, integral to membrane, transferase activity

Spezifität: Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 31.6kDa according to NP_079119. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Signaltransduktion, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Atanasova, Li, Bjarnason et al.: "Duodenal ascorbate and ferric reductase in human iron deficiency." in: The American journal of clinical nutrition, Vol. 81, Issue 1, pp. 130-3, 2005 (PubMed).

Balusikova, Neubauerova, Dostalikova-Cimburova et al.: "Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess." in: Molecular and cellular biochemistry, Vol. 321, Issue 01. Feb, pp. 123-33, 2009 (PubMed).