alcohol dehydrogenase 1A (ADH1A) Antikörper

Antigen: alcohol dehydrogenase 1A (ADH1A)

Synonyme: ADH1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185331

Produktbeschreibung

Weitere Bezeichnung: pan ADH

Gen-ID: 124, 125, 126

Immunogen: STAGKVMKCKA

Format: Affinity-purified

Beschreibung: All names and symbols: pan ADH, ADH1B, ADH2, alcohol dehydrogenase IB (class I), beta polypeptide, ADH, beta subunit, aldehyde reductase, alcohol dehydrogenase 2, alcohol dehydrogenase 2 (class I), beta polypeptide, alcohol dehydrogenase 1B (class I), beta polypeptide. Official Symbol: ADH1A, B, C. RefSeq number(s): NP_000658.1, NP_000659.2, NP_000660.1

Spezifität: This antibody is expected to recognise the alpha (ADH1A, NP_ 000658), the beta (ADH1B, NP_000659) and gamma (ADH1C, NP_000660) polypeptide variants of human alcohol dehydrogenase. Human, Mouse, Rat

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 38kDa band observed in Human Liver lysates (calculated MW of 39.9kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 0.3-1.5µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Signaltransduktion, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185331 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Ikuta, Fujiyoshi, Kurachi et al.: "Molecular cloning of a full-length cDNA for human alcohol dehydrogenase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 9, pp. 2703-7, 1985 (PubMed).

Osier, Pakstis, Kidd et al.: "Linkage disequilibrium at the ADH2 and ADH3 loci and risk of alcoholism." in: American journal of human genetics, Vol. 64, Issue 4, pp. 1147-57, 2000 (PubMed).

Huang, Lin, Ko et al.: "Possible interaction of alcohol dehydrogenase and aldehyde dehydrogenase genes with the dopamine D2 receptor gene in anxiety-depressive alcohol dependence." in: Alcoholism, clinical and experimental research, Vol. 28, Issue 3, pp. 374-84, 2004 (PubMed).

Das, Devi, Pal et al.: "Effect of complex formation between Zn2+ ions and the anticancer drug mithramycin upon enzymatic activity of zinc(II)-dependent alcohol dehydrogenase." in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, Vol. 10, Issue 1, pp. 25-32, 2005 (PubMed).