Feline Leukemia Virus Subgroup C Cellular Receptor 1 (FLVCR1) Antikörper

Antigen: Feline Leukemia Virus Subgroup C Cellular Receptor 1 (FLVCR1)

Synonyme: FLVCR, Flvcr1, 9630055N22Rik, FLVCR1, si:ch211-147d7.6

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185315

Produktbeschreibung

Weitere Bezeichnung: FLVCR

Gen-ID: 28982

Immunogen: C-KTVMLSKQSESAI

Format: Affinity-purified

Beschreibung: All names and symbols: FLVCR, feline leukemia virus subgroup C cellular receptor, FLVCR protein. Official Symbol: FLVCR. RefSeq number(s): NP_054772.1. Gene ontology terms: development, integral to plasma membrane, receptor activity, transport, transporter activity

Spezifität: Human, Chimpanzee, Cat 555 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? ABIN185315 has been successfully used in WB application in the publicatioon: Rey M.A. et al. Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis. Haematologica 2008 Nov,93(11):1617-26. Epub 2008 Sep 24. PMID:18815190.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185315 (0.1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Quigley, Yang, Worthington et al.: "Identification of a human heme exporter that is essential for erythropoiesis." in: Cell, Vol. 118, Issue 6, pp. 757-66, 2004 (PubMed).