CAMP Responsive Element Modulator (CREM) Antikörper

Antigen: CAMP Responsive Element Modulator (CREM)

Synonyme: ICER, hCREM-2, MGC17881, MGC41893, MGC111110, Icer, CREM, MGC127769, MGC140070

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185300

Produktbeschreibung

Weitere Bezeichnung: CREM

Gen-ID: 1390

Immunogen: LETLKDICSPKTDY

Format: Affinity-purified

Beschreibung: All names and symbols: CREM, ICER, MGC17881, MGC41893, MGC111110, hCREM-2, cAMP responsive element modulator, hCREM 2alpha-b protein, hCREM 2beta-a protein, inducible cAMP early repressor ICER. Official Symbol: CREM. RefSeq number(s): NP_853549.1, NP_874387.1, NP_874388.1, NP_874393.1, NP_874394.1, NP_877570.1, NP_877571.1, NP_877572.1, NP_877573.1, NP_878270.1, NP_898883.1. Gene ontology terms: DNA binding, cAMP response element binding protein binding, nucleus, nucleus, nucleus, protein binding, regulation of transcription, DNA-dependent, regulation of transcription, DNA-dependent, signal transduction, transcription factor activity

Spezifität: This antibody is expected to recognise the following protein isoforms of human CREM protein: a (NP_853549.1), e (NP_874387.1), f (NP_874388.1), k (NP_874393.1), l (NP_874394.1), m (NP_877570.1), n (NP_877571.1), o (NP_877572.1), p (NP_877573.1), q (NP_878270.1), v (NP_898883.1). This antibody is not expected to cross-react with CREB protein. Human, Mouse, Rat 300, 109 aa, 121 aa, 113 aa, 121 aa, 245 aa, 182 aa, 270 aa, 207 aa, 96 aa, 221 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 30kDa band observed in human heart lysates (calculated MW of 32.5kDa according to NP_853549.1). Recommended concentration: 1-3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185300 staining (1µg/ml) of human heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Tenbrock, Juang, Tolnay et al.: "The cyclic adenosine 5'-monophosphate response element modulator suppresses IL-2 production in stimulated T cells by a chromatin-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 6, pp. 2971-6, 2003 (PubMed).