PITPNM Family Member 3 (PITPNM3) Antikörper

Antigen: PITPNM Family Member 3 (PITPNM3)

Synonyme: NIR1, CORD5, RDGBA3, MGC157740, MGC157741

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN185211

Produktbeschreibung

Weitere Bezeichnung: NIR1 / PITPNM3

Gen-ID: 83394

Immunogen: C-SWARGPPKFESVP

Format: Affinity-purified

Beschreibung: All names and symbols: NIR1, PITPNM family member 3, PYK2 N-terminal domain-interacting receptor 1, RDGBA3. Official Symbol: PITPNM3. RefSeq number(s): NP_112497

Spezifität: Human 974 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_112497. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185211 (5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publikationen

Publikationen: Lev, Hernandez, Martinez et al.: "Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2278-88, 1999 (PubMed).