A Kinase (PRKA) Anchor Protein 8 (AKAP8) Antikörper

Antigen: A Kinase (PRKA) Anchor Protein 8 (AKAP8)

Synonyme: AKAP95, DKFZp586B1222, AA673585, AU015639, 1200016A02Rik, Akap95, AKAP8, MGC165917, MGC108502

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185207

Produktbeschreibung

Weitere Bezeichnung: AKAP8 / AKAP95

Gen-ID: 10270, 56399 (mouse), 116633 (rat)

Immunogen: DQGYGGYGAWSAG-C

Format: Affinity-purified

Beschreibung: All names and symbols: AKAP8, AKAP95, DKFZp586B1222, A kinase (PRKA) anchor protein 8, A kinase anchor protein 95. Official Symbol: AKAP8. RefSeq number(s): NP_005849. Gene ontology terms: protein kinase A anchor protein activity, mitosis, signal transduction, nucleus

Spezifität: Human, Mouse, Rat 692 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: experiments gave a band at approx 100kDa in Human Liver lysate after 1µg/ml antibody staining. This band corresponds to the 95kDa band reported in literature (PMID: 10601332). This protein has a calculated MW of 76.1kDa according to NP_005849. Recommended concentration: 1-3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185207 staining (1µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Eide, Coghlan, Orstavik et al.: "Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95." in: Experimental cell research, Vol. 238, Issue 2, pp. 305-16, 1998 (PubMed).