SH3-Domain Binding Protein 1 (SH3BP1) Antikörper

Antigen: SH3-Domain Binding Protein 1 (SH3BP1)

Synonyme: 3BP-1, SH3BP1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN185183

Produktbeschreibung

Weitere Bezeichnung: SH3BP1

Gen-ID: 23616

Immunogen: C-PQPRPRSLASETN

Format: Affinity-purified

Beschreibung: All names and symbols: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981. Official Symbol: SH3BP1. RefSeq number(s): NP_061830.3

Spezifität: Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70kDa band observed in Human Lung and Kidney lysates (predicted MW of 71kDa according to NP_061830). Recommended for use at 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185183 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN185183 (0.3µg/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Publikationen

Publikationen: Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).