Membrane Protein, Palmitoylated 6 (MAGUK P55 Subfamily Member 6) (MPP6) Antikörper

Antigen: Membrane Protein, Palmitoylated 6 (MAGUK P55 Subfamily Member 6) (MPP6)

Synonyme: VAM1, p55T, PALS2, VAM-1, P55t, Pals2, MGC189400, mpp6, MGC55662, zgc:55662

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185151

Produktbeschreibung

Weitere Bezeichnung: PALS2 / VAM1 / MPP6

Gen-ID: 51678, 56524 (mouse)

Immunogen: QQVLENLTELPSS-C

Format: Affinity-purified

Beschreibung: All names and symbols: MPP6, VAM1, p55T, VAM-1, membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6), MAGUK protein p55T, VELI-associated MAGUK 1, protein associated with Lin7 2. Official Symbol: MPP6. RefSeq number(s): NP_057531.2

Spezifität: Human, Mouse, Rat

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 60kDa band observed in lysates of cell line HEK293 (calculated MW of 61.1kDa according to NP_057531.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185151 staining (0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Kamberov, Makarova, Roh et al.: "Molecular cloning and characterization of Pals, proteins associated with mLin-7." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11425-31, 2000 (PubMed).