MPP5 Antikörper (N-Term)

Produktnummer ABIN185150

Der Ziege Polyklonal anti-MPP5 Antikörper wird verwendet zum Nachweis von MPP5 in Proben von Human. Er wurde validiert für ELISA, IHC, FACS und IF.

Kurzübersicht für MPP5 Antikörper (N-Term) (ABIN185150)

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MPP5 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-MPP5 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: PALS / MPP5

Sequenz: NKASPPFPLI SNAQ

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NKASPPFPLISNAQ-C

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Small Intestine and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary testing showed a band at approx 75 kDa in Human Cerebellum lysate after 1 μg/mL antibody staining (calculated MW of 73.4 kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu MPP5

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Andere Bezeichnung: MPP5

Hintergrund: MPP5, PALS1, FLJ12615, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5), stardust (Drosophila), MAGUK p55 subfamily member 5, membrane protein, palmitoylated 5, stardust

Gen-ID: 64398

NCBI Accession: NP_071919

Pathways: Nucleotide Phosphorylation, Tube Formation, Asymmetric Protein Localization