rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) Antikörper

Antigen: rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18)

Synonyme: AI467246, D030053O22Rik, zgc:85686, wu:fc21e04, wu:fj05f11, KIAA0521, MGC15913, P114-RhoGEF

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN185123

Produktbeschreibung

Weitere Bezeichnung: P114-RHO-GEF / ARHGEF18

Gen-ID: 23370

Immunogen: C-SAKEDASKEDVIFF

Format: Affinity-purified

Beschreibung: All names and symbols: ARHGEF18, rho/rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114. Official Symbol: ARHGEF18. RefSeq number(s): NP_056133.2, NP_001124427.1

Spezifität: This antibody is expected to recognize both reported isoforms (NP_056133.2, NP_001124427.1). Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave no signal but low background in Human Skeletal Muscle and HepG2 extracts at up to 1.5µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Blomquist, Schwoerer, Schablowski et al.: "Identification and characterization of a novel Rho-specific guanine nucleotide exchange factor." in: The Biochemical journal, Vol. 352 Pt 2, pp. 319-25, 2001 (PubMed).

Terry, Zihni, Elbediwy et al.: "Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis." in: Nature cell biology, Vol. 13, Issue 2, pp. 159-66, 2011 (PubMed).