rho GTPase Activating Protein 26 (ARHGAP26) Antikörper

Antigen: rho GTPase Activating Protein 26 (ARHGAP26)

Synonyme: GRAF, OPHN1L, OPHN1L1, FLJ42530, KIAA0621, ARHGAP26, AI853435, mKIAA0621, 1810044B20Rik, 2610010G17Rik, 4933432P15Rik

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185109

Produktbeschreibung

Weitere Bezeichnung: GRAF / ARHGAP26

Gen-ID: 23092, 71302 (mouse)

Immunogen: C-KTGLIPENYVEFL

Format: Affinity-purified

Beschreibung: All names and symbols: ARHGAP26, GRAF, Rho GTPase activating protein 26 , KIAA0621, FLJ42530, OPHN1L, OPHN1L1, GTPase regulator associated with FAK, GTPase regulator associated with focal adhesion kinase pp125(FAK), GTPase regulator associated with the focal adhesion kinase pp125. Official Symbol: ARHGAP26. RefSeq number(s): NP_055886.1. Gene ontology terms: Rho GTPase activator activity, actin cytoskeleton organization and biogenesis, cell growth and/or maintenance, neurogenesis,

Spezifität: Human, Mouse, Rat

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 92.2kDa according to NP_055886.1). Recommended concentration: 0.3-1µg/ml. An additional strong band of unknown identity was also consistently observed at 16kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185109 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Borkhardt, Bojesen, Haas et al.: "The human GRAF gene is fused to MLL in a unique t(5;11)(q31;q23) and both alleles are disrupted in three cases of myelodysplastic syndrome/acute myeloid leukemia with a deletion 5q." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 16, pp. 9168-73, 2000 (PubMed).