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Antikörper
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anti-Anillin, Actin Binding Protein (ANLN) (C-Term) Antikörper
Anillin, Actin Binding Protein (ANLN) (C-Term) Antikörper
| Antigen |
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| Synonyme |
scra, Scraps, DKFZp779A055, RGD1566097, ANLN, C78101, 1110037A17Rik, 2900037I21Rik, scraps, ABP8, Ani, Scra, anillin, l(2)03427, l(2)43Ec, DmelCG2092, CG2092 |
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Epitope
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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Durchflusszytometrie (FACS) (17), Immunfluoreszenz (IF) (17), Western Blot (WB) (13), Enzyme Immunoassay (EIA) (5), ELISA (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Immunpräzipitation (IP) (3), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1), Immunhistochemie (IHC) (1)
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| Produktnummer |
ABIN185086 |
| Menge |
100 ug (Varianten) |
| Preis |
286,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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ANILLIN / Scraps (C Terminus)
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Gen-ID
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54443, 68743 (mouse)
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Immunogen
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WQPDACYKPIGKP
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Format
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Affinity-purified
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Beschreibung
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All names and symbols: Scraps, ANILLIN, ANLN, anillin, actin binding protein (scraps homolog, Drosophila), anillin (Drosophila Scraps homolog), actin binding protein. Official Symbol: ANLN. RefSeq number(s): NP_061155.2. Gene ontology terms: actin binding, regulation of exit from mitosis, septin assembly and septum formation
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Spezifität
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Human, Mouse
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Applikationshinweise
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Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration, 3-10µg/ml.
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Reinigung
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Lagerung
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Oegema, Savoian, Mitchison et al.: "Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis." in: The Journal of cell biology, Vol. 150, Issue 3, pp. 539-52, 2000 (PubMed).
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Alternativen zu Antigen "Anillin, Actin Binding Protein (ANLN)", Typ "Antikörper" finden
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Wirte
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Kaninchen (25), Ziege (9), Maus (1)
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Reaktivitäten
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Human (33), Maus (22), Ratte (Rattus) (13), Rind (Kuh) (8), Hund (8), Schwein (3), Huhn (2), Kaninchen (2), Pferd (1), Zebrafisch (Brachydanio rerio) (1)
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Applikationen
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Durchflusszytometrie (FACS) (17), Immunfluoreszenz (IF) (17), Western Blot (WB) (13), Enzyme Immunoassay (EIA) (5), ELISA (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Immunpräzipitation (IP) (3), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1), Immunhistochemie (IHC) (1)
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Konjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (3), internal (2)
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Alternativen 
