Sorting Nexin 26 (SNX26) (C-Term) Antikörper

Antigen: Sorting Nexin 26 (SNX26)

Synonyme: TCGAP, FLJ39019, NOMA-GAP, SNX26, Snx26, AW324378, tcgap

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN184975

Produktbeschreibung

Weitere Bezeichnung: SNX26 / TCGAP (C Terminus)

Gen-ID: 115703, 233071 (mouse)

Immunogen: SWSLHSEGQTRSYC

Format: Affinity-purified

Beschreibung: All names and symbols: SNX26, TCGAP, FLJ39019, sorting nexin 26, likely ortholog of mouse TC10/CDC42 GTPase-activating protein. Official Symbol: SNX26. RefSeq number(s): NP_443180.2

Spezifität: Human, Mouse 1126 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 75kDa band in Human Bone Marrow, Kidney, and Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Brain shows cytoplasm staining of Purkinje cells in Cerebellum. Recommended concentration, 2.5µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184975 (2.5µg/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publikationen

Publikationen: Teasdale, Loci, Houghton et al.: "A large family of endosome-localized proteins related to sorting nexin 1." in: The Biochemical journal, Vol. 358, Issue Pt 1, pp. 7-16, 2001 (PubMed).