»
Antikörper
»
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) Antikörper
Checkpoint with Forkhead and Ring Finger Domains (CHFR) Antikörper
| Antigen |
|
| Synonyme |
CHFR, RNF116, RNF196, FLJ10796, FLJ33629, chfr, fc43f10, wu:fc43f10, MGC95316, rnf116, rnf196, MGC75959, DKFZp469H0121, C230082M18, 5730484M20Rik |
| Klonalität |
Polyklonal |
|
Wirt
|
|
|
Reaktivität
|
|
|
Applikation
|
|
| Produktnummer |
ABIN184918 |
| Menge |
100 ug |
| Preis |
286,00 € Zzgl. Versandkosten €40,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 5 Werktagen |
|
Weitere Bezeichnung
|
CHFR
|
|
Gen-ID
|
55743, 231600 (mouse), 288734 (rat)
|
|
Immunogen
|
KFNHICEQTRFKN
|
|
Format
|
Affinity-purified
|
|
Beschreibung
|
All names and symbols: CHFR, checkpoint with forkhead and ring finger domains, FLJ10796. Official Symbol: CHFR. RefSeq number(s): NP_060693
|
|
Spezifität
|
Human, Mouse, Rat
|
|
Applikationshinweise
|
Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5µg/ml.
|
|
Reinigung
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Lagerung
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Forschungsgebiet
|
Krebs, Zellzyklus
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
|
Publikationen
|
Scolnick, Halazonetis: "Chfr defines a mitotic stress checkpoint that delays entry into metaphase." in: Nature, Vol. 406, Issue 6794, pp. 430-5, 2000 (PubMed).
|
Alternativen zu Antigen "Checkpoint with Forkhead and Ring Finger Domains (CHFR)", Typ "Antikörper" finden
|
Wirte
|
Kaninchen (26), Maus (6), Ziege (4)
|
|
Reaktivitäten
|
Human (34), Maus (21), Ratte (Rattus) (6), Rind (Kuh) (4), Huhn (3), Hund (3), Schwein (1)
|
|
Applikationen
|
Durchflusszytometrie (FACS) (18), Immunfluoreszenz (IF) (18), Western Blot (WB) (15), ELISA (7), Immunhistochemie (Paraffinschnitte) (IHC (p)) (7), Enzyme Immunoassay (EIA) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunpräzipitation (IP) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Konjugate
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitope
|
AA 401-548 (1)
|
Alternativen 
