N-Myc Downstream Regulated Gene 1 (NDRG1) Antikörper

Antigen: N-Myc Downstream Regulated Gene 1 (NDRG1)

Synonyme: GC4, RTP, DRG1, NDR1, NMSL, TDD5, CAP43, CMT4D, HMSNL, RIT42, TARG1, PROXY1, Ndr1, Ndrl, ndrg1, ndr1, MGC53143, NDRG1, gc4, rtp, drg1, nmsl, tdd5, cap43, cmt4d, hmsnl, ndrg4, rit42, targ1, proxy1, xndrg1, MGC89705, MGC127533, cb775, MGC63944, zgc:63944, wu:fb60h02

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN184872

Produktbeschreibung

Weitere Bezeichnung: NDRG1

Gen-ID: 10397, 17990 (mouse)

Swiss-Prot: NP_006087.2

Immunogen: Peptide with sequence GNSAGPKSMEVSC, from the C Terminus of the protein sequence according to NP_006087.2, NP_001128714.1.

Kreuzreaktivität: Human, Maus, Ratte (Rattus), Hund

Format: Liquid

Beschreibung: Principal Names: tunicamycin-responsive protein, reducing agents and tunicamycin-responsive protein, protein regulated by oxygen-1, nickel-specific induction protein Cap43, differentiation-related gene 1 protein, N-myc downstream regulated gene 1 protein, TDD5, TARG1, RTP, RIT42, PROXY1, NMSL, NDR1, HMSNL, GC4, DRG1, CMT4D, CAP43,, N-myc downstream regulated gene 1, NDRG1. Official Symbol: NDRG1

Spezifität: Human, Mouse 394 aa

Molekulargewicht: 42.8kDa

Synonyme: GC4, RTP, DRG1, NDR1, NMSL, TDD5, CAP43, CMT4D, HMSNL, RIT42, TARG1, PROXY1, Ndr1, Ndrl, ndrg1, ndr1, MGC53143, NDRG1, gc4, rtp, drg1, nmsl, tdd5, cap43, cmt4d, hmsnl, ndrg4, rit42, targ1, proxy1, xndrg1, MGC89705, MGC127533, cb775, MGC63944, zgc:63944, wu:fb60h02

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kDa band observed in lysates of Human Brain, Kidney and Testis (calculated MW of 42.8kDa according to NP_006087.2). Recommended concentration: 0.1-0.3µg/ml. A minor band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. IHC: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cyrtoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration, 0.5-2µg/ml.

Konzentration: 100µg/200µl

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Krebs, Angiogenese, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184872 staining (0.1µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Zhou, Salnikow, Costa: "Cap43, a novel gene specifically induced by Ni2+ compounds." in: Cancer research, Vol. 58, Issue 10, pp. 2182-9, 1998 (PubMed).