Junction Plakoglobin (JUP) Antikörper

Antigen: Junction Plakoglobin (JUP)

Synonyme: DP3, PDGB, PKGB, CTNNG, DPIII, ARVD12, dp3, pdgb, pkgb, ctnng, dpiii, MGC75867, MGC137835, plak

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN184775

Produktbeschreibung

Weitere Bezeichnung: Plakoglobin / Gamma-catenin

Gen-ID: 3728, 16480 (mouse)

Immunogen: C-GLRPPYPTADHMLA

Format: Affinity-purified

Beschreibung: All names and symbols: GAMMA-CATENIN, gamma-catenin, JUP, junction plakoglobin, DP3, PDGB, PKGB, DPIII. Official Symbol: JUP. RefSeq number(s): NP_002221, NP_068831. Gene ontology terms: cytoplasm, cell adhesion, soluble fraction, membrane fraction, cytoskeletal protein binding protein

Spezifität: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1. Human, Mouse

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 85kDa band observed in A431 lysate (predicted MW of 86kDa according to NP_002221, NP_068831). Recommended for use at 0.2-1µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184775 staining (0.2µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Knudsen, Wheelock: "Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin." in: The Journal of cell biology, Vol. 118, Issue 3, pp. 671-9, 1992 (PubMed).