Caspase Recruitment Domain Family, Member 16 (CARD16) Antikörper

Antigen: Caspase Recruitment Domain Family, Member 16 (CARD16)

Synonyme: COP, COP1, PSEUDO-ICE

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN184661

Produktbeschreibung

Weitere Bezeichnung: COP1 / PSEUDO-ICE (Isofom 2)

Gen-ID: 114769

Immunogen: C-ETLGLSAGPIPGN

Format: Affinity-purified

Beschreibung: All names and symbols: COP, CARD only protein, PSEUDO-ICE, COP1, caspase-1 dominant-negative inhibitor pseudo-ICE, pseudo interleukin-1beta converting enzyme. Official Symbol: COP1. RefSeq number(s): NP_443121.1. Gene ontology terms: apoptosis, intracellular, apoptosis regulator activity

Spezifität: This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068, NP_001007233.1). Human 97 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave an approx 28-30kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.7kDa according to NP_443121.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Druilhe, Srinivasula, Razmara et al.: "Regulation of IL-1beta generation by Pseudo-ICE and ICEBERG, two dominant negative caspase recruitment domain proteins." in: Cell death and differentiation, Vol. 8, Issue 6, pp. 649-57, 2001 (PubMed).