Sec61 alpha 1 Subunit (S. Cerevisiae) (SEC61A1) Antikörper

Antigen: Sec61 alpha 1 Subunit (S. Cerevisiae) (SEC61A1)

Synonyme: SEC61, HSEC61, SEC61A, Sec61a, AA408394, AA410007, sec61a1, MGC53497, MGC69436, SEC61A1, secY1, Sec61a1, ACYPI000055, DKFZp469H0814

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Maus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN184559

Produktbeschreibung

Weitere Bezeichnung: SEC61A1

Gen-ID: 29927

Swiss-Prot: NP_037468.1

Immunogen: Peptide with sequence C-KEQSEVGSMGALLF, from the C Terminus of the protein sequence according to NP_037468.1.

Kreuzreaktivität: Human, Hund, Rind (Kuh), Schwein

Format: Liquid

Grade: Serum-free

Beschreibung: Official Symbol: SEC61A1

Spezifität: Human, Dog, Cow, Pig

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 48kDa band observed in Human Brain (Cerebelum) lysates (calculated MW of 52.3kDa according to NP_037468.1). Recommended concentration: 0.3-1µg/ml.

Konzentration: 100µg/200µl

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN184559 staining (3µg/ml) of Daudi lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publikationen

Publikationen: Goerlich, Prehn, Hartmann et al.: "A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation." in: Cell, Vol. 71, Issue 3, pp. 489-503, 1992 (PubMed).