Interleukin 17A (IL17A) Antikörper (Biotin)

Antigen: Interleukin 17A (IL17A)

Synonyme: IL17, CTLA8, IL-17, IL-17A, Il17, Ctla8, Ctla-8, IL17F, IL17A, ChIL-17

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Konjugat: Biotin

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN181570

Produktbeschreibung

Produktmerkmale: Synonyms: IL-17A, IL17, IL-17, CTLA8, CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8

Weitere Bezeichnung: Interleukin-17A (IL17A)

Gen-ID: 3605

Swiss-Prot: Q16552

Immunogen: Highly pure (> 98 %) recombinant human IL-17A

Beschreibung: IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originallycloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and arat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts, the enhancement of surfaceexpression of ICAM 1 in fibroblasts, activation of NF kappaB and costimulation of T cellproliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. Theprecursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 aminoacids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 showsapproximately 62. 5% amino acid homology to mouse IL17 and 58% amino acid homologyto rat IL17.

Spezifität: This antibody detects Interleukin-17A. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: ELISA: Direct: To detect hIL-17A by direct ELISA (using 100 μl/well antibody solution) aconcentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction withcompatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Sandwich: To detect hIL-17A by sandwich ELISA (using 100 μl/well antibody solution) aconcentration of 0.25 - 1.0 μg/ml is required. In conjunction with Polyclonal Anti-HumanIL-17A as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.

Buffer: PBS, pH 7.2Label: BiotinReconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 %BSA to a concentration of 0.1 - 1.0 mg/ml.

Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeksat2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeatedfreezing and thawing. Shelf life: One year from despatch.

Forschungsgebiet: Zytokine, Rezeptoren, Inflammation, Immunologie, Virologie, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar