Small Subunit H of Psii (Psbh) Antikörper

Antigen: Small Subunit H of Psii (Psbh)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Balsam fir (Abies balsamea), Arabidopsis thaliana, Barley (Hordeum vulgare), Eastern White Pine (Pinus strobus), Spinat (Spinacia oleracea), Synechococcus PCC 7942

Applikation: Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN173362

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide chosen from PsbH protein of Arabidopsisthaliana P56780

Format: Lyophilized

Beschreibung: The PsbH protein was originally named 10- or 9-kDa phosphoprotein in higherplant chloroplasts. It is encoded by the plastome in algae and higher plants. PsbHis also present in cyanobacteria, where it exhibits 56% amino acid identity with thecorresponding protein from Arabidopsis. The protein contains 63–90 amino acids,depending on the species, with molecular masses between 7.0 and 9.9 kDa.PsbH is an intrinsic membrane protein with a single transmembrane helix and itsN-terminal region has been suggested to be exposed to the stromal side of thethylakoid membrane. Presence of PsbH already present in etiolated tissue canindicate that the protein may be involved in early stages of PSII assembly.Obtained biochemical data from PSII complexes isolated from spinach suggestthat PsbH, together with other PSII phosphoproteins, may be required for D1protein turnover by regulating dimeric and monomeric PSII transition through theirphosphorylation and dephosphorylation.

Spezifität: Predicted reactivity: dicots including: Glycine max, Pisum sativum, monocots including: Oryza sativa,trees: Picea sitchensis, Populus deltoides.

Anwendungen

Applikationshinweise: Recommended Dilution 1 : 5000 with standard ECL (WB).

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

From left to right: Arabidopsis thaliana, Horderum vulgare, Chlamydmononas reinhardtii, Synechococcus sp. 7942 (2 ug of total cellular protein was loaded per lane) Antibodies have also reactivity to thylakoids from: spinach, maize and conifers (data not presented).

Publikationen

Publikationen: Zghidi-Abouzid, Merendino, Buhr et al.: "Characterization of plastid psbT sense and antisense RNAs." in: Nucleic acids research, Vol. 39, Issue 13, pp. 5379-87, 2011 (PubMed).