B-Cells (Subset) Antikörper (Biotin)

Antigen: B-Cells (Subset)

Klonalität: Monoklonal (BB6-10A10)

Wirt: Maus

Reaktivität: Schwein

Konjugat: Biotin

Produktnummer: ABIN135490

Produktbeschreibung

Weitere Bezeichnung: B Cell Subset

Isotyp: IgM

Klon: BB6-10A10

Beschreibung: Indeterminate MAb BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood, 22-23% in mesenteric lymph nodes, 79-87% in ileal Peyer's patches (ILPP), 6-17 in spleen, and 0% in thymus.1 While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by BB6-10A10 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.

Anwendungen

Applikationshinweise: Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells),

Lagerung: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Denham, Zwart, Whittall et al.: "Monoclonal antibodies putatively identifying porcine B cells." in: Veterinary immunology and immunopathology, Vol. 60, Issue 3-4, pp. 317-28, 1998 (PubMed).