H-2Kk Antikörper

Antigen: H-2Kk

Klonalität: Monoklonal (16-3-22-4)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN135434

Produktbeschreibung

Immunogen: C3H.SW mouse splenocytes

Format: Purified

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 16-3-22-4

Beschreibung: An epitope on mouse H-2Kk MHC class I alloantigen MAb 16-3-22S reacts with the H-2Kk MHC class I alloantigen. Cross-reactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis.2 The antibody does not react with other (e.g., b, d, q) haplotypes.

Anwendungen

Applikationshinweise: Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, investigators are advised to determine the optimum working dilution of the product that is appropriate for a specific need.

Lagerung: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Beschränkungen: Nur für Forschungszwecke einsetzbar