Ratte anti-Maus IgE Antikörper (Biotin)

Antigen: IgE

Klonalität: Monoklonal (23G3)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Biotin

Applikation: ELISA, Immunfluoreszenz (IF)

Produktnummer: ABIN135038

Produktbeschreibung

Isotyp: IgG1

Klon: 23G3

Beschreibung: Reacts with the heavy chain of BALB/c mouse IgE as demonstrated by ELISA.

Anwendungen

Applikationshinweise: Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells),ELISA: Alkaline phosphatase (1:500-1:1,000), Horseradish peroxidase (1:1,000-1:2,000), Biotin (1:5,000-1:10,000). Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs.

Lagerung: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8C or long-term at -20C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8C or long-term at -20C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Forschungsgebiet: Immunologie, Sekundärantikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar