Ziege Immunoglobulin G (IgG) Antikörper (PE)

Antigen: Immunoglobulin G (IgG)

Klonalität: Polyklonal

Wirt: Ziege

Konjugat: PE

Produktnummer: ABIN129766

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin G

Immunogen: Rat IgG whole molecule

Format: IgG-fraction, Liquid (sterile filtered)

Beschreibung: Ti-RAT IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rb and Sh Serum Proteins. Label Description R-Phycoerythrin (RPE). Color: Red. Label: MW 240,000. Conjugation Reference: Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversible protein-protein conjugation. Biochem J 173, 723-737 (1978). Conjugation Chemistry: SATA/MBS. Excitation Wavelength: 488. Emission Wavelength: 575.

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Anwendungen

Applikationshinweise: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. All assays should be optimized by the user. Recommended dilutions (if any) may be listed below. IFMICROSCOPY 1:100 - 1:250. OTHER ASSAYS FLOW 1:100 - 1:250.

Konzentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565

Reinheit: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide.

Lagerung: Store vial at 4° C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Beschränkungen: Nur für Forschungszwecke einsetzbar