Glutathione Peroxidase 4 (GPX4) Antikörper

Antigen: Glutathione Peroxidase 4 (GPX4)

Synonyme: MCSP, PHGPx, snGPx, snPHGPx, Phgpx, gpx-4, snGpx, cb563, cb691, PHGPxa, sb:cb563, zgc:92253, wu:fb82e03, mtPHGPx, MGC103187, MGC118087, 1700027O09Rik, F11L15.5, GLUTATHIONE PEROXIDASE 4

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129715

Produktbeschreibung

Gen-ID: CAB42657

Swiss-Prot: O70325

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.

Format: Liquid (sterile filtered)

Beschreibung: Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions as an antioxidant and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation. GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress. GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters. GPx4 is the only GPx to use phospholipid hydroperoxides as substrates. It can also reduce thymine hydroperoxide. In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione. GPx4 is expressed in high levels in testes, suggesting a role in male fertility. It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.

Spezifität: This affinity purified antibody is directed against mouse GPx4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with GPx4 protein from mouse, rat and guinea pig based on 100% homology with the immunizing sequence. Cross-reactivity with human GPx4 is also predicted based on a 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:7,000 - 1:30,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19 kDa in size corresponding to GPx4 protein by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.15 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar