Ring Finger Protein 25 (RNF25) Antikörper

Antigen: Ring Finger Protein 25 (RNF25)

Synonyme: AO7, FLJ13906

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129713

Produktbeschreibung

Weitere Bezeichnung: RNF25 (RING Finger Protein 25)

Gen-ID: NP_071898

Swiss-Prot: Q96BH1

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

Format: Liquid (sterile filtered)

Beschreibung: RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF- kappa B by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).

Spezifität: This affinity purified antibody is directed against human RNF25 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. The antibody also recognizes mouse RNF25 protein. Reactivity against homologues from other sources has not been determined.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54 kDa in size corresponding to RNF25 protein by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Ionenkanäle

Beschränkungen: Nur für Forschungszwecke einsetzbar