Death-Domain Associated Protein (DAXX) Antikörper

Antigen: Death-Domain Associated Protein (DAXX)

Synonyme: DAP6, EAP1, BING2, MGC126245, MGC126246, MGC150289, DAP-3, 4921514D13Rik, im:6905684, LOC100226911

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129710

Produktbeschreibung

Weitere Bezeichnung: Death domain-associated protein 6 (DAXX)

Gen-ID: NP_001341

Swiss-Prot: Q9UER7

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 261-274 of human DAXX protein.

Format: Liquid (sterile filtered)

Beschreibung: DAXX, the Death domain-associated protein 6 (also named Fas death domain-associated protein, ETS1-associated protein 1 or EAP1), functions in part as a transcriptional co-repressor through its interaction with a number of nuclear, DNA-associated proteins, such as histone deacetylase II, core histones H2A, H2B, H3 and H4, and Dek, a chromatin-associated protein reported to change the topology of DNA in chromatin in vitro (1). DAXX also interacts with p73, and is involved in the regulation of the cell cycle and apoptosis through transcriptional regulation of p53 and possibly its family members (2).

Spezifität: This affinity-purified antibody is directed against human DAXX protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with DAXX protein from human, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:7,000 to 1:45,000 WESTERN BLOT 1:200 to 1:2000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to DAXX protein by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:7,000 to 1:45,000 WESTERN BLOT 1:200 to 1:2000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 1.06 mg/ml (by UV absorbance at 280)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Beschränkungen: Nur für Forschungszwecke einsetzbar