Epithelial Cell Transforming Sequence 2 Oncogene (ECT2) Antikörper

Antigen: Epithelial Cell Transforming Sequence 2 Oncogene (ECT2)

Synonyme: FLJ10461, MGC138291, ECT2, ect2, xect2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129688

Produktbeschreibung

Weitere Bezeichnung: ECT2 pT790 (Epithelial Cell Transforming Sequence 2 Oncogene)

Gen-ID: NP_060568

Swiss-Prot: Q9H8V3

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein. Protein Sequence: Human ECT2, 883 aa, predicted MW 100.0 kDa 1 maensvltst tgrtsladss ifdskvteis kenlligsts yveeempqie trvilvqeag 61 kqeelikalk dikvgfvkme sveefeglds pefenvfvvt dfqdsvfndl ykadcrvigp 121 pvvlncsqkg eplpfscrpl yctsmmnlvl cftgfrkkee lvrlvtlvhh mggvirkdfn 181 skvthlvanc tqgekfrvav slgtpimkpe wiykawerrn eqdfyaavdd frnefkvppf 241 qdcilsflgf sdeektnmee mtemqggkyl plgdercthl vveenivkdl pfepskklyv 301 vkqewfwgsi qmdaragetm ylyekantpe lkksvsmlsl ntpnsnrkrr rlketlaqls 361 retdvspfpp rkrpsaehsl sigslldisn tpessinygd tpksctkssk sstpvpskqs 421 arwqvakely qtesnyvnil atiiqlfqvp leeegqrggp ilapeeikti fgsipdifdv 481 htkikddled livnwdesks igdiflkysk dlvktyppfv nffemsketi ikcekqkprf 541 haflkinqak pecgrqslve llirpvqrlp svalllndlk khtadenpdk stlekaigsl 601 kevmthined krkteaqkqi fdvvyevdgc panllsshrs lvqrvetisl gehpcdrgeq 661 vtlflfndcl eiarkrhkvi gtfrsphgqt rppaslkhih lmplsqikkv ldiretedch 721 nafallvrpp teqanvllsf qmtsdelpke nwlkmlcrhv antickadae nliytadpes 781 fevntkdmds t lsrasraik ktskkvtraf sfsktpkral rralmtshgs vegrspssnd 841 khvmsrlsst sslagipsps lvslpsffer rshtlsrstt hli

Format: Liquid (sterile filtered)

Beschreibung: ECT2, also known as epithelialcell transforming sequence 2 oncogene, was originallyisolated as a transforming gene from epithelial cells. ECT2catalyzes guanine nucleotide exchange on the smallGTPases, RhoA, Rac1, and Cdc42. ECT2 may be phos-phorylated during G 2 and M phases, and phosphorylation may be required for its exchange activity. Unlike otherknown guanine nucleotide exchange factors for RhoGTPases, ECT2 exhibits nuclear localization ininterphase, spreads throughout the cytoplasm inprometaphase, and is condensed in the midbody duringcytokinesis. Expression of dominant-negative ECT2 ormicroinjection of affinity-purified anti-ECT2 antibody intointerphase cells strongly inhibits cytokinesis. These resultssuggest that ECT2 is an important link between the cellcycle machinery and Rho signaling pathways involved inthe regulation of cell division. Phosphorylation at T790 orS375 significantly affects the catalytic activity of ECT2.

Spezifität: This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein atthe pT790 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserumwas first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was thencross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human ECT2protein at the pT790 residue. By ELISA the antibody is specific for the phosphorylated form of the protein. Reactivity withnon-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates,which are predicted to contain non-phosphorylated ECT2. Additional experiments are underway to clarify antibody specificity.A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee,chicken, and dog based on 100% homology with the immunizing sequence. Reactivity against homologues from othersources is not known. ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:1,000 - 1:4,000 IF MICROSCOPY User Optimized OTHER APPLICATION User Optimized

Anwendungen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by westernblotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form ofthe immunizing peptide. This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue.Experiments to generate more conclusive evidence are currently under preparation.

Konzentration: 1.31 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Beschränkungen: Nur für Forschungszwecke einsetzbar