Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) Antikörper

Antigen: Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F)

Synonyme: EIF3S5, eIF3-p47, EIF3F, Eif3s5, AA409853, MGC90606, 0610037M02Rik, LOC733116, eIF3f, eIF3-S5

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129687

Produktbeschreibung

Weitere Bezeichnung: Eukaryotic Translation Initiation Factor 3 (eIF3f)

Swiss-Prot: O00303

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 114-125 of human eIF3f protein.

Format: Liquid (sterile filtered)

Beschreibung: EIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.

Spezifität: This affinity-purified antibody is directed against human eIF3f protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with eIF3f protein from human, mouse, rat, chimpanzee, chicken, and bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against eIF3f from Drosophila melanogaster and Tetraodon nigroviridis (pufferfish) based on 90% homology of the immunizing sequence with eIF3f from these sources. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATION User Optimized

Konzentration: 0.78 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Translationsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Wada, Kato, Okutsu et al.: "Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy." in: The Journal of biological chemistry, 2011 (PubMed).