REC107 Antikörper

Antigen: REC107

Synonyme: MER2

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129681

Produktbeschreibung

Weitere Bezeichnung: S.cervosoae Mer2

Swiss-Prot: P21651

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.

Format: Liquid (sterile filtered)

Beschreibung: Mer2 (also known asmeiotic recombination 2 protein) is a chromosomalprotein that is critical for meiotic recombination andprogression. It is phosphorylated at two serineresidues, S30 and S271 by the yeast Cdk1 cyclin-dependent kinase homolog. This phosphorylationis S-phase specific, and thus has the potential tobe a specific assay for S-phase cyclin-dependentkinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that S-phase CDK associates with thereplication fork.

Spezifität: This affinity-purified antibody is directed against the Saccharomyces cerevisiae Mer2 protein.The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs againstSaccharomyces cerevisiae Mer2 protein and reactivity is independent of phosphorylation at residue S30. A BLASTanalysis was used to suggest minimal cross reactivity with Mer2 homologues from other sources.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in sizecorresponding to Mer2 protein by western blotting in the appropriate cell lysate or extract. This antibody is reactive withboth phosphorylated and unphosphorylated Mer2 at the S30 position. Dilutions: ELISA 15,000 - 125,000 WESTERN BLOT 11,000 - 110,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 0.75 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior toopening. Dilute only prior to immediate use. Forextended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing andthawing. Expiration date is one (1) year from dateof opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar