F-Box Protein (FBXO) Antikörper

Antigen: F-Box Protein (FBXO)

Synonyme: HREP, Fbw10, SM25H2, SM2SH2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129674

Produktbeschreibung

Weitere Bezeichnung: F-Box Only Protein

Gen-ID: NP_036479 (isoform 1), NP_258441 (isoform 2), NP_258442 (isoform 3)

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding to amino acids 431-447 of human FBOX9 protein. Protein Sequence: Human FBOX9, 447 aa, predicted MW 52.3 kDa 1 mpdiiwvfpp qaeaeedchs dtvradddee nespaetdlq aqlqmfraqw mfelapgvss 61 snlenrpcra argslqktsa dtkgkqeqak eekarelflk aveeeqngal yeaikfyrra 121 mqlvpdiefk itytrspdgd gvgnsyiedn dddskmadll syfqqqltfq esvlklcqpe 181 lessqihisv lpmevlmyif rwvvssdldl rsleqlslvc rgfyicardp eiwrlaclkv 241 wgrsciklvp ytswremfle rprvrfdgvy iskttyirqg eqsldgfyra whqveyyryi 301 rffpdghvmm lttpeepqsi vprlrtrntr tdaillghyr lsqdtdnqtk vfavitkkke 361 ekpldykyry frrvpvqead qsfhvglqlc ssghqrfnkl iwihhschit ykstgetavs 421 afeidkmytp lffarvrsyt afserpl

Format: Liquid (sterile filtered)

Beschreibung: F-box only protein 9(also called FBOX9 and Fbp9) is a member of theF-box protein family, which is characterized by anapproximately 40 amino acid motif, the F-box. TheF-box proteins constitute one of the four subunitsof the ubiquitin protein ligase complex called SCFs(SKP1-cullin-F-box), which function inphosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbwscontaining WD-40 domains, Fbls containingleucine-rich repeats, and Fbxs containing eitherdifferent protein-protein interaction modules or norecognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the5' terminus. Isoform 1 is distinct from isoforms 2 and 3 at the N-terminus.

Spezifität: This affinity-purified antibody is directed against human FBOX9 protein. The product wasaffinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggestcross reactivity with FBOX9 protein from human, dog, rat and chimpanzee based on 100% homology with theimmunizing sequence. Expect partial reactivity with homologues from zebrafish, bovine and mouse (94% homology) aswell as chicken (88%) and Xenopus laevis (82%). Reactivity is expected against all known isoforms of this protein, asthe immunogen sequence is common to their carboxy-terminal ends. Reactivity against homologues from othersources is not known.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Although the predicted MW of FBOX9 isoform 1 is 52kDa, antibody reactivity against MCF7 whole cell lysates shows a predominant band at 100 kDa. This band is believedto be FBOX9 and the higher apparent MW may be due to its association with other proteins. Isoforms 1, 2 and 3 havereported molecular weights of 52.3, 51.1 and 47.3 kDa, respectively. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Although the predicted MW of FBOX9 isoform 1 is 52kDa, antibody reactivity against MCF7 whole cell lysates shows a predominant band at 100 kDa. This band is believedto be FBOX9 and the higher apparent MW may be due to its association with other proteins. Isoforms 1, 2 and 3 havereported molecular weights of 52.3, 51.1 and 47.3 kDa, respectively. Dilutions: ELISA 1:5,000 - 1:25,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATION User Optimized

Konzentration: 1.53 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior toopening. Dilute only prior to immediate use. Forextended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing andthawing. Expiration date is one (1) year from dateof opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar