Histone H2Av (0) (pS137) Antikörper

Antigen: Histone H2Av (0)

Synonyme: H2A.F/Z, H2A.X, H2A.Z, H2AV, H2AX, H2AZ, H2AvD, HIS2AV, HIS2AVD, HisH2Av, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602, Dmel\CG5499, CG5499

Epitope: pS137

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129671

Produktbeschreibung

Weitere Bezeichnung: Histone H2AvD pS137

Gen-ID: NP_524519

Swiss-Prot: P08985

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein. Protein Sequence Drosophila melanogaster H2AvD pS137, 140 aa, predicted MW 14.8 kDa 1 maggkagkds gkakakavsr saraglqfpv grihrhlksr ttshgrvgat aavysaaile 61 yltaevlela gnaskdlkvk ritprhlqla irgdeeldsl ikatiagggv iphihkslig 121 kkeetvqdpq rkgnvil s qa y

Format: Liquid (sterile filtered)

Beschreibung: "Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a corecomponent of the nucleosome, an octamer containing two moleculeseach of H2A, H2B, H3 and H4. The octamer wraps approximately146 bp of DNA. HsAvD is expressed both maternally and zygoticallyand is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ""knock out"" mice have an increased incidence of cancer."

Spezifität: This affinity-purified antibody is directed against the phosphorylated form of Drosophila H2AvD proteinat the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserumwas first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was thencross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila H2AvDpS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylatedDrosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvDproteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions forreactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylatedH2AvD protein by western blotting in the appropriate Drosophilatissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.This antibody is phospho specific for pS137 of H2AvD protein. Dilutions: ELISA 1:4,000 - 1:16,000 WESTERN BLOT 1:400 - 1:1,600 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 1.09 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Forschungsgebiet: DNS/RNS, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar