CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) Antikörper

Antigen: CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL)

Synonyme: CTCFL, Boris, RP23-44L6.1, OTTMUSG00000016680, BORIS, CT27, CTCF-T, MGC163358, dJ579F20.2, AW108038, CTCF, im:7152666, xctcf

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129668

Produktbeschreibung

Weitere Bezeichnung: BORIS

Gen-ID: AAM28645

Swiss-Prot: Q8NI51

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding aa 9-26 of human BORIS protein.

Format: Liquid (sterile filtered)

Beschreibung: BORIS (Brother of the Regulatorof Imprinted Sites) also known as CCCTC-binding factor-likeprotein, is normally only expressed in the testis and expressedin a mutually exclusive manner with CTCF during male germcell development. However, previous studies have shown thatBORIS is abnormally activated in a wide range of humancancers. Expression of BORIS in normally BORIS-negativecells promotes cell growth that may lead to transformation.BORIS maps to the cancer-associated amplification regionthought to contain an oncogene or dominant-immortalizinggene. BORIS is a candidate protein for the epigeneticreprogramming factor acting in the male germ line. BORIS isfound in both the nucleus and cytoplasm.

Spezifität: This affinity-purified antibody is directed against the human BORIS protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivitywith BORIS proteins from human and chimpanzee. Partial reactivity may occur against BORIS from mouse, dog and ratsources based on varying degrees of homology to the immunizing sequence. Reactivity against homologues from othersources is not known.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in sizecorresponding to BORIS by western blotting in the appropriate cell lysate or extract. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:200 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 1.28 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening.Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles offreezing and thawing. Expiration date is one (1) year from dateof opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Dougherty, Ichim, Liu et al.: "Selective apoptosis of breast cancer cells by siRNA targeting of BORIS." in: Biochemical and biophysical research communications, Vol. 370, Issue 1, pp. 109-12, 2008 (PubMed).

Fiorentino, Macaluso, Miranda et al.: "CTCF and BORIS Regulate Rb2/p130 Gene Transcription: A Novel Mechanism and a New Paradigm for Understanding the Biology of Lung Cancer." in: Molecular cancer research : MCR, Vol. 9, Issue 2, pp. 225-33, 2011 (PubMed).