NADPH Oxidase Organizer 1 (NOXO1) Antikörper

Antigen: NADPH Oxidase Organizer 1 (NOXO1)

Synonyme: SNX28, P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5, MGC20258, hslt, Snx28, 2310034C04Rik, NOXO1, cb18, sb:cb18, wu:fd09d09, noxo1, snx28, p41nox, p41noxa, p41noxb, p41noxc, sh3pxd5

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129665

Produktbeschreibung

Weitere Bezeichnung: NOXO1

Gen-ID: AAH15917

Swiss-Prot: Q96B73

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 238-252 of human NOXO1 protein.

Format: Liquid (sterile filtered)

Beschreibung: The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells. NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region. NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.

Spezifität: This affinity purified antibody is directed against human NOXO1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from human and chimpanzee (100% homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from dog and guinea pig (87%), as well as rat (75%) and mouse (68%). Reactivity against homologues from other sources is not known.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:20,000 - 1:100,000 WESTERN BLOT 1:2,000 - 1:8,000 IMMUNOHISTOCHEMISTRY 5-10 ug/ml OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract.

Konzentration: 1.17 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Signaltransduktion, Metabolismus, Transkriptionsfaktoren, Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-NADPH Oxidase Organizer 1 (NOXO1) antibody

1.17 mg/ml (by UV absorbance at 280 nm)