Slit Homolog 3 (Drosophila) (SLIT3) Antikörper

Antigen: Slit Homolog 3 (Drosophila) (SLIT3)

Synonyme: MEGF5, SLIL2, SLIT1, slit2, Slit-3, FLJ10764, Slil2, MGC146100, zgc:111911, Megf5, SLIT3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129655

Produktbeschreibung

Weitere Bezeichnung: SLIT-3

Gen-ID: NP_003053

Swiss-Prot: O75094

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding aa 1164-1177 of Human SLIT-3 protein.

Format: Liquid (sterile filtered)

Beschreibung: SLIT-3 (also known as multiple epidermal growth factor-like domain 5 and Slit homolog 3 protein) is a Slit protein. The 'slit'gene has been shown to play a critical role in central nervous systemmidline formation. In addition to SLIT3 there are two additional human'slit' homologs, which are termed SLIT1 and SLIT2. Each SLIT geneencodes a putative secreted protein, which contains conservedprotein-protein interaction domains including leucine-rich repeats andepidermal growth factor-like motifs, similar to those of the Drosophilaprotein. SLIT proteins may also participate in the formation andmaintenance of the nervous and endocrine systems by protein-proteininteractions. Slit-3 is a secreted protein predominantly expressed inthyroid. Multiple isoforms have been reported for this product.

Spezifität: This affinity purified antibody is directed against human SLIT-3 protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with thisprotein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect crossreactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within theimmunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bandscorresponding to isoforms of SLIT-3 by western blotting in theappropriate cell lysate or extract. Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 0.83 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Beschränkungen: Nur für Forschungszwecke einsetzbar