Voltage-Dependent Anion Channel 1 (VDAC1) Antikörper

Antigen: Voltage-Dependent Anion Channel 1 (VDAC1)

Synonyme: PORIN, MGC111064, PORIN-31-HL, Vdac5, AL033343, fa13f11, zgc:85830, wu:fa13f11, MGC53435, vdac1, VDAC1

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129654

Produktbeschreibung

Weitere Bezeichnung: VDAC/Porin

Swiss-Prot: P21796

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 185-197 of Human VDAC1/Porin1.

Format: Liquid (sterile filtered)

Beschreibung: VDAC (also known as Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL) is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger - see below). The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mito-chondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations.

Spezifität: This affinity purified antibody is directed against human VDAC1/Porin1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:10,000 - 1:40,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract.

Konzentration: 0.4 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Signaltransduktion, Metabolismus, Organelle, Apoptose/Nekrose

Beschränkungen: Nur für Forschungszwecke einsetzbar