Diaphanous Homolog 2 (Drosophila) (DIAPH2) Antikörper

Antigen: Diaphanous Homolog 2 (Drosophila) (DIAPH2)

Synonyme: DIAPH2, Diap2, RGD1564399, DIAPH1, MGC148327, DIA, POF, DIA2, DRF2, POF2, mDia3, FLJ11167, DIA3, E430022I22Rik, dia2, sb:eu1025

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129631

Produktbeschreibung

Weitere Bezeichnung: DIA-2

Gen-ID: NP_006720

Swiss-Prot: O60879

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizationswith a synthetic peptide corresponding aa 1085-1101 of Human DIA-2. Protein Sequence: Human DIA2, 1101 aa, predicted MW 125.6 kDa 1 meqpgaaasg agggseepgg grsnkrsagn raaneeetkn kpklniqikt laddvrdrit 61 sfrkstvkke kpliqhpids qvamsefpaa qplydersln lsekevldlf ekmmedmnln 121 eekkaplrnk dfttkremvv qyisataksg glknskhect lssqeyvhel rsgisdekll 181 ncleslrvsl tsnpvswvnn fgheglglll deleklldkk qqenidkknq ykliqclkaf 241 mnnkfglqri lgdersllll araidpkqpn mmteivkils aicivgeeni ldkllgaitt 301 aaernnrerf spiveglenq ealqlqvacm qfinalvtsp yeldfrihlr neflrsglkt 361 mlpdlkeken deldiqlkvf denkeddlte lshrlndira emddmnevyh llynmlkdta 421 aenyflsilq hfllirndyy irpqyykiie ecvsqivlhc sgmdpdfkyr qrldidlthl 481 idscvnkakv eeseqkaaef skkfdeefta rqeaqaelqk rdekikelea eiqqlrtqaq 541 vlssssgipg ppaapplpgv gppppppapp lpggaplppp ppplpgmmgi pppppppllf 601 ggpppppplg gvppppgisl nlpygmkqkk mykpevsmkr inwskiepte lsencfwlrv 661 kedkfenpdl faklalnfat qikvqknaea leekktgptk kkvkelrild pktaqnlsif 721 lgsyrmpyed irnvilevne dmlsealiqn lvkhlpeqki lnelaelkne yddlcepeqf 781 gvvmssvkml qprlssilfk ltfeehinni kpsiiavtla ceelkksesf nrllelvllv 841 gnymnsgsrn aqslgfkinf lckirdtksa dqkttllhfi adiceekyrd ilkfpeeleh 901 vesaskvsaq ilksnlasme qqivhlerdi kkfpqaenqh dkfvekmtsf tktareqyek 961 lstmhnnmmk lyenlgeyfi fdsktvsiee ffgdlnnfrt lfleavrenn krremeektr 1021 raklakekae qeklerqkkk kqlidinkeg detgvmdnll ealqsgaafr drrkriprnp 1081 dnrrvplers rsrhngaiss k

Format: Liquid (sterile filtered)

Beschreibung: DIA-2 (also called DIAdrome, Dia2, DIAPH2, Diaphanous 2, Diaphanousrelated formin 2, Diaphorase 2, DRF2, POF and POF2)may play a role in the development and normal functionof the ovaries. Mutations of this gene have been linkedto premature ovarian failure. Alternative splicing resultsin two protein isoforms. Furthermore, each splicevariant undergoes additional splicing in the 3' UTR.Overall 4 splice variants have been described. DIA-2 isexpressed in testis, ovary, small intestine, prostate,lung, liver, kidney and leukocytes and can be foundfrom E16 in ovary and testis and during P6-P16 duringdifferentiation of ovarian follicles. Isoform 1 is referredto as DIA-156, whereas isoform 2 is called DIA-12C.

Spezifität: This affinity purified antibody is directed against human DIA-2. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivitywith this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity isexpected with mouse DIA-2 based on a 93% homology to the immunogen sequence. Cross reactivity with DIA-2homologues from other sources has not been determined. ELISA 1:12,000 - 1:60,000 WESTERN BLOT 1:1,000 - 1:6,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:12,000 - 1:60,000 WESTERN BLOT 1:1,000 - 1:6,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in sizecorresponding to DIA-2 by western blotting in the appropriate cell lysate or extract. Alternate splice variants have beendescribed for this protein. This antibody will only react with DIA-156 (isoform 1 of DIA-2).

Konzentration: 1.4 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Diaphanous Homolog 2 (Drosophila) (DIAPH2) antibody