Insulin Receptor Substrate 1 (IRS1) (pS307) Antikörper

Antigen: Insulin Receptor Substrate 1 (IRS1)

Synonyme: HIRS-1, G972R, IRS-1, IRS1IRM, irs1, MGC75645, IRS1

Epitope: pS307

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129580

Produktbeschreibung

Weitere Bezeichnung: IRS1 pS307

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 298-316 of Human IRS1 (insulin receptor substrate-1, a substrate of the insulin receptor kinase). Human IRS1 acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors. Peptide sequence: G-L-T-R-R-S-R-T-E-pS-I-T-A-T-S-P-A-S-M

Format: Liquid (sterile filtered)

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product has been assayed by ELISA against 0.1 ug of the immunizing peptide. A 1:2,000 to 1:4,000 dilution of the antibody is recommended for this assay. Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.

Konzentration: 0.76 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Signaltransduktion, Wachstumsfaktoren, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Shah, Hunter: "Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6425-34, 2006 (PubMed).