Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1) Antikörper

Antigen: Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1)

Synonyme: ADTG, CLAPG1, MGC18255, SYNG, AP1GBP1, FLJ34482, MGC104959, Adtg, AA409002, AU041323, AW551707, D8Ertd374e, adtg, zgc:56079, wu:fc30a11, AP1G1, DKFZp469F1514, ap1g1, MGC122095

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Maus, Ratte (Rattus), Schaf

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129554

Produktbeschreibung

Weitere Bezeichnung: AP1G1 (gamma 1 Subunit of Adaptor-Related Protein Complex 1)

Gen-ID: NP_001119

Swiss-Prot: O43747

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizationswith a synthetic peptide corresponding aa 646-659 of Human AP1G1. Protein Sequence: Human AP1G1, 825 aa, predicted MW 91.7 kDa 1 mpapirlrel irtirtartq aeeremiqke caairssfre edntyrcrnv akllymhmlg 61 ypahfgqlec lkliasqkft dkrigylgam lllderqdvh llmtnciknd lnhstqfvqg 121 lalctlgcmg ssemcrdlag evekllktsn sylrkkaalc avhvirkvpe lmemflpatk 181 nllneknhgv lhtsvvllte mcerspdmla hfrkslqlvp qlvrilknli msgyspehdv 241 sgisdpflqv rilrllrilg rndddsseam ndilaqvatn tetsknvgna ilyetvltim 301 diksesglrv lainilgrfl lnndkniryv altsllktvq tdhnavqrhr stivdclkdl 361 dvsikrrame lsfalvngnn irgmmkelly fldscepefk adcasgifla aekyapskrw 421 hidtimrvlt tagsyvrdda vpnliqlitn svemhaytvq rlykailgdy sqqplvqvaa 481 wcigeygdll vsgqceeeep iqvtedevld ilesvlisnm stsvtrgyal taimklstrf 541 tctvnrikkv vsiygssidv elqqraveyn alfkkydhmr sallermpvm ekvttngpte 601 ivqtngetep apletkppps gpqptsqand lldllggndi tpviptapts kpssaggell 661 dllgdinltg apaaapapas vpqisqppfl ldglssqplf ndiaagipsi taysknglki 721 eftfersntn psvtvitiqa snsteldmtd fvfqaavpkt fqlqllspss sivpafntgt 781 itqvikvlnp qkqqlrmrik ltynhkgsam qdlaevnnfp pqswq

Format: Liquid (sterile filtered)

Beschreibung: AP1G1 (also known as gamma 1 subunit of adaptor -related protein complex 1, Gamma-adaptin, Adaptor protein complex AP-1 gamma-1 subunit, Golgi adaptor HA1/AP1 adaptin gamma-1 subunit andClathrin assembly protein complex 1 gamma-1 large chain) is a subunit of clathrin-associated adaptor protein complex 1that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexesmediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails oftransmembrane cargo molecules. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form aheterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles.AP1G1 is localized to the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex and iswidely expressed.

Spezifität: This affinity purified antibody is directed against human AP1G1. The product was affinitypurified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with thisprotein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Crossreactivity with AP1G1 protein from mouse, rat, dog and chicken may occur as sequence homology varies by only oneamino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP1G1 proteins from othersources is not known.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:2,000 - 1:8,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specificconditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in sizecorresponding to AP1G1 by western blotting in the appropriate cell lysate or extract.

Konzentration: 0.7 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storagealiquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) yearfrom date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using antibodies-online's Affinity Purified anti-AP1G1 antibody shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.