gamma 1 Adaptin Antikörper (AA 625-675)

Produktnummer ABIN129554

Hersteller Produkt- Nr.: 600-101-289

Der Ziege Polyklonal anti-gamma 1 Adaptin Antikörper wird verwendet zum Nachweis von gamma 1 Adaptin in Proben von Human. Er wurde validiert für WB, ELISA und IP.

Kurzübersicht für gamma 1 Adaptin Antikörper (AA 625-675) (ABIN129554)

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser gamma 1 Adaptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-gamma 1 Adaptin Antikörper

Bindungsspezifität: AA 625-675

Hersteller Produkt- Nr.: 600-101-289

Hersteller: Rockland

Verwendungszweck: AP1G1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human AP1G1.

Produktmerkmale: Synonyms: goat anti-AP1G1 Antibody, AP-1 complex subunit gamma-1, Adapter-related protein complex 1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, Gamma1-adaptin, Clathrin assembly protein complex 1 gamma-1 large chain, ADTG, CLAPG1, AP1G

Aufreinigung: The product was affinity purified from antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 625-675 of Human AP1G1.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to AP1G1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:8,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.7 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu gamma 1 Adaptin

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Andere Bezeichnung: AP1G1

Hintergrund: Background: AP1G1 (also known as gamma 1 subunit of adaptor -related protein complex 1, Gamma-adaptin, Adaptor protein complex AP-1 gamma-1 subunit, Golgi adaptor HA1/AP1 adaptin gamma-1 subunit and Clathrin assembly protein complex 1 gamma-1 large chain) is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.  The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins.  Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles.  AP1G1 is localized to the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex and is widely expressed.

Gen-ID: 164, 71772942

UniProt: O43747