Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1) Antikörper

Antigen: Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1)

Synonyme: ADTAA, CLAPA1, AP2-ALPHA, hip9, hypj, adtab, ap2a1, clapa2, MGC69489, AP2A1, adtaa, clapa1, ap2-alpha

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Maus, Ratte (Rattus), Schaf

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129553

Produktbeschreibung

Weitere Bezeichnung: AP2A1 alpha-1 subunit of adaptor-related protein complex 2)

Gen-ID: NP_055018 (alpha 1), NP_036437 (alpha 2)

Swiss-Prot: O95782

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with asynthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins

Format: Liquid (sterile filtered)

Beschreibung: The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly proteincomplex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit.The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin(sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.

Spezifität: This affinity purified antibody is directed against human AP2A. The product was affinity purified fromantiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with AP2A1 and AP2A2 protein fromhuman, chimpanzee, orangutan, mouse, rat, chicken and dog based on 100% homology for the immunogen sequence. Crossreactivity with AP2A proteins from other sources is not known.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions forreactivity should be optimized by the end user. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide. Dilutions: ELISA 1:5,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Konzentration: 1.7 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contentsand freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using antibodies-online's Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.