ULP1 (ULP1) Antikörper

Antigen: ULP1 (ULP1)

Synonyme: NIB1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Hefe (Saccharomyces Cerevisiae)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129522

Produktbeschreibung

Weitere Bezeichnung: Ubiquitin-Like Protein-Specific Protease 1 (ULP1)

Gen-ID: NP_015305

Swiss-Prot: Q02724

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1protein.

Format: Liquid (sterile filtered)

Beschreibung: ULP-1, ubiquitin-like protein-specific protease 1, initially processes Smt3 and also acts as adeconjugating enzyme for Smt3 [Saccharomyces cerevisiae (Baker's yeast)]. Covalent modification of cellular proteins by theubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signaltransduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins fordegradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Oncecovalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization andstability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans.Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified invertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. Three distinct steps can be distinguished in the SUMOmodification pathway: 1) activation of SUMO, 2) transfer of SUMO to the conjugating enzyme, and 3) substrate modification. Since SUMO is synthesized as a precursor protein, a maturation step precedes the activation reaction. In yeast, C-terminal processing of the SUMO precursor is mediated by the processing protease Ulp1, which has an additional role in the deconjugation of SUMO-modified substrates. Mature SUMO is activated by SUMO-activating enzyme, an E 1 -like heterodimeric protein complex composed of Uba2 and Aos1. Ulp1 function has provided evidence that SUMO modification in yeast, as has been suspected for vertebrates, plays an important role in nucleocytoplasmic trafficking.

Spezifität: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process whichincludes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer statedabove. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined. Protein Sequence: Yeast ULP-1, 621 aa, predicted MW 72.4 kDa 1 msvevdkhrn tlqyhkknpy splfspisty rcyprvlnnp sesrrsasfs giykkrtnts 61 rfnylndrrv lsmeesmkdg sdraskagfi ggiretlwns gkylwhtfvk neprnfdgse 121 veasgnsdve srssgsrssd vpyglrenys sdtrkhkfdt stwalpnkrr riesegvgtp 181 stspisslas qksncdsdns itfsrdpfgw nkwktsaigs nsenntsdqk nsydrrqygt 241 afirkkkvak qninntklvs raqseevtyl rqifngeykv pkilkeerer qlklmdmdke 301 kdtglkksii dltekiktil iennknrlqt rnendddlvf vkekkissle rkhkdylnqk 361 lkfdrsilef ekdfkrynei lnerkkiqed lkkkkeqlak kklvpelnek dddqvqkala 421 srentqlmnr dnieitvrdf ktlaprrwln dtiieffmky iekstpntva fnsffytnls 481 ergyqgvrrw mkrkktqidk ldkiftpinl nqshwalgii dlkkktigyv dslsngpnam 541 sfailtdlqk yvmeeskhti gedfdlihld cpqqpngydc giyvcmntly gsadapldfd 601 ykdairmrrf iahliltdal k General

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:4,000 - 1:20,000 WESTERN BLOT 1:500 - 1:2,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized. Note: This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimizedby the end user. Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriatelysate or extract.

Konzentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extendedstorage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completelyclear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior toimmediate use. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Proteasen, Aminosäuren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using antibodies-online's Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4

Western blot using antibodies-online's Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 ug of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4