Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) Antikörper

Antigen: Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B)

Synonyme: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Klonalität: Polyklonal

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129516

Produktbeschreibung

Weitere Bezeichnung: BUBR1 Kinase

Gen-ID: NP_001202

Swiss-Prot: O60566

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinantprotein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein. Hybridoma: Produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Format: Liquid (sterile filtered)

Isotyp: IgG1  (Passende Sekundärantikörper)

Beschreibung: BUBR1 Kinase (also called Mitotic checkpoint serine/threonine-protein kinase, BUB1 beta, MAD3/BUB1-relatedprotein kinase, Mitotic checkpoint kinase MAD3L and SSK1) is a probable component of the mitotic checkpoint that delaysanaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F,CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, itcan be localized to nuclear kinetochores. This protein is highly expressed in thymus followed by spleen. Defects in BUB1Bare associated with tumor formation.

Spezifität: This Protein A purified antibody is directed against human BUBR1 Kinase protein. The product waspurified from tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivityagainst homologues from other sources is not known.

Synonyme: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Anwendungen

Applikationshinweise: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescencestaining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should beoptimized by the end user. Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting inthe appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein.The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slipsfixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4containing 0.1% BSA. Dilutions: WESTERN BLOT 1:200 - 1:1,000 IMMUNOPRECIPITATION User Optimized IF MICROSCOPY 1:200 - 1:1,000 FLOW CYTOMETRY User Optimized

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date ofopening.

Forschungsgebiet: Kinasen/Phosphatasen

Beschränkungen: Nur für Forschungszwecke einsetzbar