D2 Protein of Psii (Psbd) Antikörper

Antigen: D2 Protein of Psii (Psbd)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Barley (Hordeum vulgare), Chlamydomonas reinhardtii (C. reinhardtii), Synechococcus PCC 7942, Cyanobacterium Anabaena sp. PCC 7120

Applikation: Western Blot (WB)

Produktnummer: ABIN125883

Produktbeschreibung

Immunogen: KLH-comjugated synthetic peptide derived from the C-terminal of known PsbDsequences including Arabidopsis pumila A4QJS8 , Hordeum vulgare P11849,Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898

Format: Lyophilized

Beschreibung: D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as aheterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein,with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 proteinis an important step in the assemply of the PSII reaction centre complex.

Spezifität: Predicted reactivity: dicots including: Pisum sativum, Solanum tuberosum, monocots including: Oryzasativa, Zea mays, trees: Pinus thunbergii, Populus trichocarpa, moss:Physcomitrella patens, algae.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 5000 with standard ECL (WB). Additional Information: Perfect across all full-length PsbD sequences from higher plants, lowerplants, cyanobacteria and unicellular algae except: -minor substitutions in someProchlorococcus & Dinoflagellate sequences. The antibody should still workagainst these taxa, but it has not been tested yet. This antibody does not detectPsbA protein (D1).

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

From left to right: Arabidopsis thaliana, Horderum vulgare, Chlamydmononas reinhardtii, Synechococcus sp. 7942, Anabaena 7120 (2 ug of total cellular protein was loaded per lane)

Publikationen

Publikationen: Latijnhouwers, Xu, Muoller: "Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development." in: Planta, 2010 (PubMed).

Perreault, Dionne, Didur et al.: "Effect of cadmium on photosystem II activity in Chlamydomonas reinhardtii: alteration of O-J-I-P fluorescence transients indicating the change of apparent activation energies within photosystem II." in: Photosynthesis research, 2010 (PubMed).